What are proteomic assays?
Proteomic analysis (proteomics) refers to the systematic identification and quantification of the complete complement of proteins (the proteome) of a biological system (cell, tissue, organ, biological fluid, or organism) at a specific point in time.
What is a characterization assay?
Generally, ADA characterization assay consists of two different assays, namely titering testing, and isotype analysis. Titering assays are used to characterize the magnitude of the ADA response. Whereas, the goal of isotype assays is to detect all relevant immunoglobulin (Ig) isotypes.
What is a targeted assay?
Targeted assays are a valuable tool for rapidly and accurately establishing pharmacodynamics, translational studies, gauging efficacy and safety, and post-market surveillance studies.
What is targeted proteomics?
An emerging area in the field of proteomics is targeted proteomics, a technique concerned with the quantification of specific proteins. This method has high accuracy, reproducibility, and multiplexing capabilities.
What is proteomic tool?
Proteomic tools measure gene expression, protein activity and interactions of biological events at the protein level. This will further advance our knowledge of how biological systems are built up and what mechanisms control these systems.
What is characterization of cell lines?
Cell line characterization is critical to the control of biological product manufacturing. The objective of the testing is to confirm the identity, purity, and suitability of the cell substrate for manufacturing use.
What is cellular characterization?
Cell characterization is a process of analyzing a circuit using static and dynamic methods to generate models suitable for chip implementation flows.
What assay is used to identify proteins?
There are two methods that are commonly used to identify proteins: Edman Degradation and Mass Spectrometry. Developed by Pehr Edman, Edman Degradation is a method of sequencing amino acids in a peptide.
What is target engagement assay?
Target engagement (TE) assays measure compound binding at select target proteins in intact cells. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.
What is discovery proteomics?
The purpose of discovery proteomics is to gather information about all proteins and protein forms in a biological sample. With only little previous knowlegde of the sample, discovery proteomics can identify thousands of proteins and protein forms in a single experiment.
What is global proteomics?
Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology.
What kind of cancer can be found in cptac?
CPTAC has provided the Genomic Data Commons (GDC) with genomic data from a total of 1100+ cancer patients with diverse disease types including Endometrial, Renal, Lung Adenocarcinoma and Squamous Cell Carcinoma, Breast, Colon, Ovarian, Brain, Head and Neck, and Pancreatic cancers.
Where can I find cptac harmonized genomic data?
The CPTAC harmonized genomic data is available in the GDC Data Portal. CPTAC makes proteomic data that are processed through the CPTAC Common Data analysis Pipeline (CDAP) available in the CPTAC Data Portal. CPTAC proteomic data is also available in the Proteomic Data Commons (PDC). The CPTAC genomic data can be found on the GDC Data Portal.
What did cptac do for proteogenomics research?
CPTAC’s progress in proteogenomics enabled the creation of two programs in 2016 aimed at deepening our understanding of this emerging field.
What is the purpose of the clinical proteomic tumor Analysis Consortium?
CPTAC is a comprehensive and coordinated effort to accelerate the understanding of the molecular basis of cancer through the application of robust, quantitative, proteomic technologies and workflows. The overarching goal of CPTAC is to improve our ability to diagnose, treat and prevent cancer.