What is a Fynomer?
Background: Fynomers are small binding proteins (7 kDa) derived from the SH3 domain of Fyn kinase. They can be engineered to yield specific and high-affinity binding domains to target proteins of interest.
Is protein an organic compound?
A protein is an organic molecule composed of amino acids linked by peptide bonds. While carbohydrates and lipids are composed of hydrocarbons and oxygen, all proteins also contain nitrogen (N), and many contain sulfur (S), in addition to carbon, hydrogen, and oxygen.
How do antibodies bind to proteins?
With protein antigens, the antibody molecule contacts the antigen over a broad area of its surface that is complementary to the surface recognized on the antigen. Electrostatic interactions, hydrogen bonds, van der Waals forces, and hydrophobic interactions can all contribute to binding.
What organic compounds are found in the body?
Most organic compounds making up our cells and body belong to one of four classes: carbohydrates, lipids, proteins, and nucleic acids. These molecules are incorporated into our bodies with the food we eat.
How are fynomers used as a binding protein?
Fynomers are small binding proteins (7 kDa) derived from the human Fyn SH3 domain, which can be engineered to bind to essentially any target of interest with high affinity and specificity (for a review on non-immunoglobulin binding proteins collectively called “scaffolds” (see Refs. 11 and 12 ).
How are fynomers engineered to bind to targets?
Fynomers are small 7-kDa globular proteins derived from the SH3 domain of the human Fyn kinase (Fyn SH3) that can be engineered to bind with antibody-like affinity and specificity to virtually any target of choice through random mutation of two loops (RT- and src-loop) on the surface of the Fyn SH3 domain ( 13–15 ).
What are fynomers and how are they used in research?
This person is not on ResearchGate, or hasn’t claimed this research yet. Fynomers are small binding proteins derived from the human Fyn SH3 domain. Using phage display technology, Fynomers were generated inhibiting the activity of the proinflammatory cytokine interleukin-17A (IL-17A).
Which is fynomer inhibits the activity of IL-17A?
Fynomers are small binding proteins derived from the human Fyn SH3 domain. Using phage display technology, Fynomers were generated inhibiting the activity of the proinflammatory cytokine interleukin-17A (IL-17A). One specific Fynomer called 2C1 inhibited human IL-17A in vitro with an IC50 value of 2.2 nm.