Are Daphnia affected by caffeine?
Both caffeine and dopamine were found to instantly increase the heart rate of Daphnia in a dose-dependent manner. Interestingly, caffeine synergized with dopamine to increase Daphnia’s heart rate.
What is the effect of caffeine on the heart rate of Daphnia?
What is the effect of caffeine alcohol and nicotine on the heart rate of Daphnia magna?
Our raw data showed that the addition of caffeine and nicotine in the solution that the Daphnia Magna resides in, caused an increase in heart rate while the addition of ethanol caused a decrease in heart rate.
Does nicotine increase Daphnia heart rate?
The 25% nicotine solution caused the heart rate to decrease at a steady pace for one minute and then drastically decrease. Multiple trials found that nicotine causes a decrease in heart rate for Daphnia magna, with higher concentrations of nicotine having a stronger effect.
What affects Daphnia heart rate?
Daphnia is poikilothermic, which means that its body temperature and therefore its metabolic rate are affected directly by the temperature of the environment. The change in metabolic rate is reflected in the rate at which the heart beats (cardiac frequency).
Do Daphnia have a brain?
The Daphnia’s nervous system consists of a brain that has two or three pairs of ganglia. Ganglia are nerve cells that tend to cluster up. The nervous system also has nerve rings that go round the oesophagus. Last of all, it has a paired ventral nerve cord.
How does caffeine increase heart rate?
Caffeine and Your Heart Caffeine in high doses raises your blood level of epinephrine. Epinephrine is also known as adrenalin. In pure forms, epinephrine can increase blood pressure, increase the contractility or force of the heart, and mildly increase the heart rate.
How does caffeine affect heart rate experiment?
Contrary to the hypothesis, caffeine did not increase the heart rate; however, analysis of the data showed a slight decrease in heart rate over increasing caffeine concentration.
Why does Daphnia heart rate decrease with caffeine?
We hypothesized that introducing ethanol will decrease the heart rate because this is a depressant which will lower their adrenaline concentration. Alternatively, introducing caffeine will increase the heart rate because it is a stimulant which changes the levels of the neurotransmitter Epinephrine (adrenaline).
Does CBD water increase Daphnia heart rate?
CBD, the misleading stimulant: No effect of Daphnia magna heart rate.
Does caffeine increase heart rate experiment?
How does caffeine affect the heart rate of Daphnia?
It is predicted that a higher concentration of caffeine the daphnia are subjected to will increase the heart rate more than compared to a solution of a lower caffeine concentration. The change in metabolic rate will be reflected in the cardiac frequency. Method 1.
How long to leave Daphnia in caffeine solution?
Time to acclimatise – leave Daphnia in caffeine solution for same amount of time to acclimatise (e.g. 5 minutes) Daphnia are most likely not complex enough to suffer physical and mental stress. Nevertheless, there is still debate over whether or not animals should possess rights as humans do.
How to calculate the heart rate of a Daphnia?
Dab around the Daphnia with a tissue to remove the pond water and replace with drops of caffeine solution (e.g. 0.1M). Leave the Daphnia for 5 minutes to acclimatise and then observe & count (using a counter) the heart rate under the microscope for 30 seconds (multiply number by 2 to calculate beats per minute).
Which is better for heart rate Daphnia or brine shrimp?
Daphnia is better than Brine shrimp because brine shrimp is not transparent meaning heart rate has to be measured by counting leg movement. Variables: The independent variable that we change.