How does fluorescence plate reader work?
The emission system of the plate reader uses polarizing filters to analyze the polarity of the emitted light. A low level of polarization indicates that small fluorescent molecules move freely in the sample. A high level of polarization indicates that fluorescent is attached to a larger molecular complex.
How does a microplate work?
A microplate reader detects light signals produced by samples which have been pipetted into a microplate. Microplate-based measurements detect light signals produced by a sample, converted by a sample or transmitted through a sample. The signal is measured by a detector, usually a photomultiplier tube (PMT).
How fluorescence is measured in a fluorescence plate reader?
Fluorescence plate readers are usually intended for fluorescence intensity and FRET detection. Consequently, fluorescence plate readers measure the light signal emitted by a sample in Relative Fluorescent Units (RFU).
What is a SpectraMax?
The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors.
What is SoftMax pro?
SoftMax® Pro Software for Windows 10 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting.
What are microtiter strips?
Microplate strips are strips of 8 microplate wells that fit into plastic plate frames, enabling custom plate configurations with up to 96 wells per plate frame. Microplate strips are available with different well capacities, well shapes, and surface coatings, depending on the application.
How does an ELISA plate reader work?
ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein. Chemical dyes fluoresce or emit one color or wavelength when exposed to light.
What is SoftMax software?
What do you read on a microtiter plate?
Microplate readers read biological, chemical or physical events that occur on a microtiter plate.
What makes a microplate reader a multi mode reader?
A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP).
What do the spectramax M series microplate readers measure?
Configurable readers with triple-mode cuvette ports, validation tools, and compliance software The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF.
What kind of software does spectramax i3x microplate reader use?
The SpectraMax i3x Multi-Mode Microplate Reader and SoftMax Pro Software provide greater flexibility than other systems. We have been working with Molecular Devices for over 15 years and we appreciate the technical quality of the instruments as well as the control and analysis software.