What does the 35S promoter gene do?
The promoter of the 35S RNA is a very strong constitutive promoter responsible for the transcription of the whole CaMV genome. It is well known for its use in plant transformation. It causes high levels of gene expression in dicot plants.
Is the CaMV 35S promoter constitutive?
The 35S CaMV promoter is generally considered to be a strong constitutive promoter1 and it facilitates high level of RNA transcription in a wide variety of plants, including plants well outside the host range of the virus2.
What is a ubiquitin promoter?
Plant ubiquitin (Ubi-1) promoter (with untranslated region intron) driving expression of a synthetic, optimised GUSPlus gene (CAMBIA) followed by an NOS terminator. The GUSPlus gene is used as a reporter gene for quantitative promoter expression in plant tissues.
Does RNA have a promoter?
Promoter. A promoter is a sequence of DNA needed to turn a gene on or off. The process of transcription is initiated at the promoter. Usually found near the beginning of a gene, the promoter has a binding site for the enzyme used to make a messenger RNA (mRNA) molecule.
What is nopaline synthase?
Nopaline synthase is an enzyme which catalyses the synthesis of nopaline, an opine which is formed as the result of the condensation of the amino acid arginine and alpha-ketoglutaric acid. The type of opine produced is specific to the particular strain of A. tumefaciens.
How do you prevent cauliflower mosaic virus?
There is no cure for cauliflower mosaic virus. Once a plant is infected, then it is too late. Prevention is the only way to prevent agricultural or gardening losses. Since cauliflower mosaic virus is usually spread by aphids, so any sort of pest control can be beneficial in reducing infection.
What does it mean to be constitutively expressed?
Definition. Constitutive expression means the continuous transcription of a gene in an organism.
What is maize ubiquitin promoter?
The maize ubiquitin 1 (Ubi-1) promoter has been widely used to drive constitutive transgene expression in monocotyledonous plants. The expression of uidA driven by this promoter depended to a great extent on the specific transformation event.
How far upstream is a promoter?
Summary. Promoters are about 100-1000 base pairs long and are adjacent and typically upstream (5′) of the sense or coding strand of the transcribed gene. The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA transcript produced.